Friday 5 December 2014

DNA FINGER PRINTING


                  The role of finger-points in forensic cases is undisputed. Based on this knowledge, that similar to fingerprints, each individual also has a unique genome with the same nucleotide sequences, scientists  developed a technique, known as DNA fingerprinting .

                 No two individuals, except for identical twins, have the same genome. Recent evidences have demonstrated that the human genome contains large families of DNA polymorphisms of many different types viz. VNTRs. These are short DNA sequences present as tandem repeats of variable length at several chromosomal locations. which can be used to produce DNA fingerprints.

                  DNA fingerprinting provides a powerful forensic tool, to nail the culprits even from minute amounts of blood, semen, hair bulbs or other cells, amplified by PCR (Poly-merase Chain Reaction) and then suitably analysed.

                Although this technique is widely used in all cases of questionable identity but they have proven specially effective in solving paternity and forensic cases, e.g., recently it has been used in identification of terrorists of 26/11 Mumbai attack, in Nithairi murdar case, in solving paternity issue of Mr. N.D. Tewari, etc.

          DNA Fingerprints These are specific banding pattern on southern blots of genomic DNA cleaved with a specific restriction enzyme and hybridised to appropriate DNA probes.

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